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human pd1 fc protein  (R&D Systems)


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    R&D Systems human pd1 fc protein
    DA treatment sensitises MDA‐MB‐231 cells to activated T cells. Representative images (A) and quantification (B) of green fluorescence‐labelled <t>PD1/Fc</t> on DA‐treated MDA‐MB‐231 cells for 24 h. Representative images (C) and quantification (D) of crystal violet‐stained live cancer cells in T cell‐mediated cancer cell killing assay. MDA‐MB‐231 cells were then co‐cultured with activated T cells for 48 h with or without DA (12.5 μM) and subjected to crystal violet staining. Statistical significance was determined using * p < 0.05 and ** p < 0.01. All error bars are expressed as mean ± SD of three independent experiments. Abbreviations: DA, dihydroartemisinin; CTRL, negative control.
    Human Pd1 Fc Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human pd1 fc protein/product/R&D Systems
    Average 96 stars, based on 78 article reviews
    human pd1 fc protein - by Bioz Stars, 2026-06
    96/100 stars

    Images

    1) Product Images from "FoxO3a‐Mediated Modulation of PD‐L1 Expression and Inhibition by Dihydroartemisinin in Triple‐Negative Breast Cancer"

    Article Title: FoxO3a‐Mediated Modulation of PD‐L1 Expression and Inhibition by Dihydroartemisinin in Triple‐Negative Breast Cancer

    Journal: Journal of Cellular and Molecular Medicine

    doi: 10.1111/jcmm.70947

    DA treatment sensitises MDA‐MB‐231 cells to activated T cells. Representative images (A) and quantification (B) of green fluorescence‐labelled PD1/Fc on DA‐treated MDA‐MB‐231 cells for 24 h. Representative images (C) and quantification (D) of crystal violet‐stained live cancer cells in T cell‐mediated cancer cell killing assay. MDA‐MB‐231 cells were then co‐cultured with activated T cells for 48 h with or without DA (12.5 μM) and subjected to crystal violet staining. Statistical significance was determined using * p < 0.05 and ** p < 0.01. All error bars are expressed as mean ± SD of three independent experiments. Abbreviations: DA, dihydroartemisinin; CTRL, negative control.
    Figure Legend Snippet: DA treatment sensitises MDA‐MB‐231 cells to activated T cells. Representative images (A) and quantification (B) of green fluorescence‐labelled PD1/Fc on DA‐treated MDA‐MB‐231 cells for 24 h. Representative images (C) and quantification (D) of crystal violet‐stained live cancer cells in T cell‐mediated cancer cell killing assay. MDA‐MB‐231 cells were then co‐cultured with activated T cells for 48 h with or without DA (12.5 μM) and subjected to crystal violet staining. Statistical significance was determined using * p < 0.05 and ** p < 0.01. All error bars are expressed as mean ± SD of three independent experiments. Abbreviations: DA, dihydroartemisinin; CTRL, negative control.

    Techniques Used: Fluorescence, Staining, Cell Culture, Negative Control



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    DA treatment sensitises MDA‐MB‐231 cells to activated T cells. Representative images (A) and quantification (B) of green fluorescence‐labelled <t>PD1/Fc</t> on DA‐treated MDA‐MB‐231 cells for 24 h. Representative images (C) and quantification (D) of crystal violet‐stained live cancer cells in T cell‐mediated cancer cell killing assay. MDA‐MB‐231 cells were then co‐cultured with activated T cells for 48 h with or without DA (12.5 μM) and subjected to crystal violet staining. Statistical significance was determined using * p < 0.05 and ** p < 0.01. All error bars are expressed as mean ± SD of three independent experiments. Abbreviations: DA, dihydroartemisinin; CTRL, negative control.
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    DA treatment sensitises MDA‐MB‐231 cells to activated T cells. Representative images (A) and quantification (B) of green fluorescence‐labelled <t>PD1/Fc</t> on DA‐treated MDA‐MB‐231 cells for 24 h. Representative images (C) and quantification (D) of crystal violet‐stained live cancer cells in T cell‐mediated cancer cell killing assay. MDA‐MB‐231 cells were then co‐cultured with activated T cells for 48 h with or without DA (12.5 μM) and subjected to crystal violet staining. Statistical significance was determined using * p < 0.05 and ** p < 0.01. All error bars are expressed as mean ± SD of three independent experiments. Abbreviations: DA, dihydroartemisinin; CTRL, negative control.
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    DA treatment sensitises MDA‐MB‐231 cells to activated T cells. Representative images (A) and quantification (B) of green fluorescence‐labelled <t>PD1/Fc</t> on DA‐treated MDA‐MB‐231 cells for 24 h. Representative images (C) and quantification (D) of crystal violet‐stained live cancer cells in T cell‐mediated cancer cell killing assay. MDA‐MB‐231 cells were then co‐cultured with activated T cells for 48 h with or without DA (12.5 μM) and subjected to crystal violet staining. Statistical significance was determined using * p < 0.05 and ** p < 0.01. All error bars are expressed as mean ± SD of three independent experiments. Abbreviations: DA, dihydroartemisinin; CTRL, negative control.
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    DA treatment sensitises MDA‐MB‐231 cells to activated T cells. Representative images (A) and quantification (B) of green fluorescence‐labelled <t>PD1/Fc</t> on DA‐treated MDA‐MB‐231 cells for 24 h. Representative images (C) and quantification (D) of crystal violet‐stained live cancer cells in T cell‐mediated cancer cell killing assay. MDA‐MB‐231 cells were then co‐cultured with activated T cells for 48 h with or without DA (12.5 μM) and subjected to crystal violet staining. Statistical significance was determined using * p < 0.05 and ** p < 0.01. All error bars are expressed as mean ± SD of three independent experiments. Abbreviations: DA, dihydroartemisinin; CTRL, negative control.
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    DA treatment sensitises MDA‐MB‐231 cells to activated T cells. Representative images (A) and quantification (B) of green fluorescence‐labelled <t>PD1/Fc</t> on DA‐treated MDA‐MB‐231 cells for 24 h. Representative images (C) and quantification (D) of crystal violet‐stained live cancer cells in T cell‐mediated cancer cell killing assay. MDA‐MB‐231 cells were then co‐cultured with activated T cells for 48 h with or without DA (12.5 μM) and subjected to crystal violet staining. Statistical significance was determined using * p < 0.05 and ** p < 0.01. All error bars are expressed as mean ± SD of three independent experiments. Abbreviations: DA, dihydroartemisinin; CTRL, negative control.
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    Image Search Results


    DA treatment sensitises MDA‐MB‐231 cells to activated T cells. Representative images (A) and quantification (B) of green fluorescence‐labelled PD1/Fc on DA‐treated MDA‐MB‐231 cells for 24 h. Representative images (C) and quantification (D) of crystal violet‐stained live cancer cells in T cell‐mediated cancer cell killing assay. MDA‐MB‐231 cells were then co‐cultured with activated T cells for 48 h with or without DA (12.5 μM) and subjected to crystal violet staining. Statistical significance was determined using * p < 0.05 and ** p < 0.01. All error bars are expressed as mean ± SD of three independent experiments. Abbreviations: DA, dihydroartemisinin; CTRL, negative control.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: FoxO3a‐Mediated Modulation of PD‐L1 Expression and Inhibition by Dihydroartemisinin in Triple‐Negative Breast Cancer

    doi: 10.1111/jcmm.70947

    Figure Lengend Snippet: DA treatment sensitises MDA‐MB‐231 cells to activated T cells. Representative images (A) and quantification (B) of green fluorescence‐labelled PD1/Fc on DA‐treated MDA‐MB‐231 cells for 24 h. Representative images (C) and quantification (D) of crystal violet‐stained live cancer cells in T cell‐mediated cancer cell killing assay. MDA‐MB‐231 cells were then co‐cultured with activated T cells for 48 h with or without DA (12.5 μM) and subjected to crystal violet staining. Statistical significance was determined using * p < 0.05 and ** p < 0.01. All error bars are expressed as mean ± SD of three independent experiments. Abbreviations: DA, dihydroartemisinin; CTRL, negative control.

    Article Snippet: Cells in each group were fixed in 4% PFA for 15 min, incubated with recombinant human PD1 Fc protein (R&D Systems, Minneapolis, MN, USA) for 1 h, and then incubated with anti‐human Alexa Fluor 488 secondary antibodies (Thermal Fisher Scientific) for 1 h at room temperature.

    Techniques: Fluorescence, Staining, Cell Culture, Negative Control